Vibrio vulnificusglycogen branching enzyme preferentially transfers very short chains: N1 domain determines the chain length transferred
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چکیده
منابع مشابه
Chain length determines the folding rates of RNA.
We show that the folding rates (k(F)s) of RNA are determined by N, the number of nucleotides. By assuming that the distribution of free-energy barriers separating the folded and the unfolded states is Gaussian, which follows from central limit theorem arguments and polymer physics concepts, we show that k(F)≈k(0)exp(-αN(0.5)). Remarkably, the theory fits experimental rates spanning over 7 order...
متن کاملCharacterization of the branching patterns of glycogen branching enzyme truncated on the N-terminus.
Truncation of 112 amino acids at the N-terminus (Nd(1-112)) changes the chain transfer pattern of the Escherichia coli glycogen branching enzyme (GBE) [Arch. Biochem. Biophys. 397 (2002) 279]. We investigated further the role of the N-terminus by engineering other truncated GBEs and analyzing the branching pattern by high-performance anion-exchange chromatography. The wild type GBE transfers ma...
متن کاملThe unique branching patterns of Deinococcus glycogen branching enzymes are determined by their N-terminal domains.
Glycogen branching enzymes (GBE) or 1,4-alpha-glucan branching enzymes (EC 2.4.1.18) introduce alpha-1,6 branching points in alpha-glucans, e.g., glycogen. To identify structural features in GBEs that determine their branching pattern specificity, the Deinococcus geothermalis and Deinococcus radiodurans GBE (GBE(Dg) and GBE(Dr), respectively) were characterized. Compared to other GBEs described...
متن کاملPersistence Length of Short-Chain Branched Polyethylene
The effect of short-chain branching (SCB) on the persistence length lp of polyethylene (PE) was studied using small-angle neutron scattering (SANS). In thermodynamically good solvents, lp can be measured directly from the scattering vector qtr at the crossover from good solvent mass-fractal scaling to the rodlike persistent scaling, using the unified equation described in the text. The method w...
متن کاملOverexpression of STARCH BRANCHING ENZYME II increases short-chain branching of amylopectin and alters the physicochemical properties of starch from potato tuber
BACKGROUND Starch is biosynthesised by a complex of enzymes including various starch synthases and starch branching and debranching enzymes, amongst others. The role of all these enzymes has been investigated using gene silencing or genetic knockouts, but there are few examples of overexpression due to the problems of either cloning large genomic fragments or the toxicity of functional cDNAs to...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 2015
ISSN: 0014-5793
DOI: 10.1016/j.febslet.2015.03.011